84-1097-88　LAX1 (Linker for Activation of X Cells) 100ug　L1440

特徴

• LAX1 (Linker for activation of X cells) is a 70kD member of a small group of T cell transmembrane (TM) adaptor proteins.
• It is upregulated on both T and B cells after activation, and likely downmodulates both BCR and TCR signaling.
• Human LAX1 is 398 amino acids (aa) in length.
• Although its predicted MW is 44kD, it runs anomalously at 70kD in SDS-PAGE.
• It is a type III (no signal sequence) TM protein that contains a 37 aa N-terminal extracellular region and a 340 aa cytoplasmic domain (aa 59-398).
• There are two potential isoform variants.
• One has an alternate start site at Met77, while a second shows a 13 aa substitution for aa 1-29.
• Over aa 136-398, human LAX1 is 47% aa identical to mouse LAX1.
• Applications:Suitable for use in Flow Cytometry, Direct ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Flow Cytometry: 10ul labels 1-5x10e5 cells in a total reaction volume ≤200ul.
• Tested using Jurkat cells.
• For intraellular staining, cells must be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS
• Direct ELISA: 0.5-1ug/ml The detection limit for rhLAX1 ~0.3ng/well
• Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.