Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine.
IL-17 binds to IL-17 receptor (IL-17 R), which shares no homology with any known family of receptors.
While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution and has been detected in virtually all cells and tissues tested.
The hIL-17 R gene was localized to chromosome 22.
Human IL-17 R is an 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail.
The aa sequence of hIL-17 R is 69% identical to the mIL-17 R.
The signaling events of IL-17 include activation of NF-kB and JNK, and require TNF receptor-associated factor 6 (TRAF6).
Applications:Suitable for use in Western Blot, Flow Cytometry and ELISA.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1ug/mlFlow Cytometry: 2.5ug/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Interleukin 17 Receptor extracellular domain. Species Crossreactivity: recombinant human IL-17(~5%). Less than 1% crossreactivity is observed with recombinant mouse IL-17 RC and recombinant mouse IL-17 RD.
Isotype：IgG
Calc Applications Abbrev：E FC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to mouse Interleukin 17 Receptor extracellular domain, expressed in Sf21 cells.
