84-1097-82　Interleukin 15 Receptor alpha (IL-15R alpha, IL-15Ra) 100ug　I8437-35E

特徴

• Applications:Suitable for use in Neutralization of Bioactivity, Direct ELISA, Western Blot and Flow Cytometry.
• Other applications not tested.
• Recommended Dilution:Neutralization of Mouse IL-15Ra Bioactivity: The exact concentration of antibody required to neutralize the mouse cell surface IL-15Ra mediated bioactivity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied.
• The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface IL-15Ra mediated IL-15 response on a responsive cell line, at a specific IL-15 concentration.
• The ND50 for this lot of anti-mouse IL-15Ra antibody was determined to be ~0.3-1.5ug/ml in the presence of 0.8ng/ml of human IL-15, using the CTLL-2 cell proliferation assay.
• Direct ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect mouse IL-15Ra.
• The detection limit for rmIL-15Ra is ~0.1ng/well.
• In this format, this antibody shows ~5% crossreactivity with rhIL-15Ra and less than 1% crossreactivity with rhIL-2Ra, rmIL-2Rb and rmIL-2Rg.
• Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse IL-15Ra.
• The detection limit for rmIL-15Ra is ~5ng/lane under non-reducing and reducing conditions.
• Flow Cytometry: 3-10ug/ml/10e6 cells with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.