Interleukin 4 is a pleiotropic cytokine produced primarily by activated T cells, mast cells and basophils.
The diverse biological effects of IL-4 on a variety of cell types are mediated by the binding of IL-4 to specific cell surface receptors.
As is the case with many other cytokines, the functional high-affinity receptor for IL-4 is a complex consisting of a ligand binding subunit and a second subunit (Beta chain) that can modulate the ligand binding affinity of the receptor complex.
It has been shown that in certain cell types, the gamma chain of the IL-2 receptor is a functional component (Beta chain) of the IL-4 receptor complex.
cDNA clones for the ligand binding chain (IL-4 R) of both the mouse and human high affinity IL-4 receptors have been isolated.
The human or mouse IL-4 R is an approximately 140kD transmembrane protein containing an extracellular domain, a transmembrane domain, and a large cytoplasmic domain that is essential for IL-4 signal transduction.
In addition to the cDNA clone encoding the full-length transmembrane protein, a second cDNA clone that arises from alternate splicing encodes a soluble secreted form of IL-4 R that has been isolated from mouse cells, but not yet from human sources.
A naturally-occurring soluble form of the IL-4 R has also been identified in mouse biological fluids and in murine cell culture supernatants.
Native or recombinant murine soluble IL-4 R, as well as recombinant human soluble IL-4 R, can bind IL-4 with the same affinity as the membrane bound IL-4 R.
Soluble IL-4 R is a competitive inhibitor of IL-4 and has been shown to effectively neutralize many IL-4-mediated responses both in vivo and in vitro.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: Neat.
Use 10ul to label 10e6 cells.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size：100Tests
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
