Human Integrin Beta7 (also Integrin BetaP and M290 IEL Ag) is a 120kD member of the Integrin beta chain family of molecules.
It is a type I transmembrane glycoprotein that contains a 704 amino acid (aa) extracellular domain (ECD) (aa20-723) and a 52aa cytoplasmic tail.
The ECD contains one von Willebrand factor A domain (aa150-389) and four cysteine-rich EGF-like repeats.
One splice variant exists that shows a deletion of aa501-648.
Integrin Beta7 forms a nondisulfide linked heterodimer with alpha4 and alphaE integrins.
Alpha4Beta7 binds fibronectin, VCAM-1 and MADCAM1, while alphaEBeta7 binds E-cadherin.
The ECD of human Beta7 is 87% aa identical to the ECD of mouse Beta7.
Applications:Suitable for use in Direct ELISA, Western Blot and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Direct ELISA: 0.5-1.0ug/ml.
The detection limit is ~0.2ng/well
Western Blot: 0.1-0.2ug/ml.
The detection limit is ~5ng/lane under non-reducing and reducing conditions
Flow Cytometry: 25ug/ml.
10ul labels 1-2.5x10e5 cells in a total reaction volume <200ul.
Tested using blood derived lymphocytes
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Integrin beta7. Shows ~20% cross-reactivity with recombinant mouse Integrin beta7 and <1% cross-reactivity with recombinant human Integrin beta2, beta5 and beta6.
Isotype：IgG
Calc Applications Abbrev：E FC WB
Calc Crossreactivity：Hu
Immunogen：CHO cell-derived recombinant human Integrin Beta7 long isoform, extracellular domain (aa20-723, Accession # P26010)
