Alpha-L-Iduronidase (IDUA) is a ubiquitously expressed lysosomal glycosyl hydrolase.
It hydrolyzes the unsulfated terminal alpha-L-Iduronic acid residues in glycosaminoglycans including dermatan sulfate and heparin sulfate.
Mutations in IDUA that result in enzymatic deficiency lead to the autosomal recessive disease mucopolysaccharidosis.
Applications:Suitable for use in Direct ELISA, Immunocytochemistry, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml Human kidney tissue and IMR human lung fibroblast cell lineImmunocytochemistry: 5-15ug/ml; immersion fixed HepG2 human hepatocellular carcinoma cell lineImmunoprecipitation: 25ug/ml; conditioned cell culture medium spiked with recombinant human IDUA
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human alpha-L-Iduronidase/IDUA.
Isotype：IgG
Calc Applications Abbrev：E IC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala26-Pro653 from human alpha-L-Iduronidase/IDUA, expressed in NSO cells.
