84-1097-71　Hepsin (TMPRSS1) 100ug　H2030-21B

特徴

• Hepsin (Transmembrane protease serine 1) is a 51kD member of the peptidase S1 family of enzymes.
• It is expressed by both hepatocytes and numerous tumor cells, and activates HGF plus pro-uPA.
• Human Hepsin is a type II transmembrane protein that is 417 amino acids (aa) in length.
• It contains a 23 aa N-terminal cytoplasmic region and a 373 aa extracellular domain (ECD) (aa 45-417).
• The ECD possesses a SRCR segment (aa 54-151) and a peptidase S1 domain (aa 163-405).
• Over aa 46-417, human Hepsin shares 90% and 96% aa identity with mouse and canine Hepsin, respectively.
• Applications:Suitable for use in Direct ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Direct ELISA: 0.5 -1.0ug/ml detection limit for rhHepsin is ~ 3ng/well
• Western Blot: 0.1-0.2ug/ml detection limit for rhHepsin is ~ 5ng/lane under non-reducing and reducing conditions
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.