Glypican 1 (glypiated proteoglycan 1) is a 200kD, highly glycosylated member of the GPI-linked Glypican family of proteins.
It is expressed by both epithelial and mesodermally-derived cells, and appears to use its proteoglycan adduct to regulate growth factor signaling and availability.
Human Glypican 1 is synthesized as a 535 amino acid (aa) proprecursor that contains a 507 mature region (aa 24-530) and a 28 aa C-terminal prosegment.
There are three potential splice variants.
One shows an alternate start site at Met73, a second incorporates the same start site with a 65 aa substitution for aa 295-558, and a third shows a deletion of aa 184-558.
Mature human Glypican 1 is 91% aa identical to mouse Glypican 1.
Applications:Suitable for use in Direct ELISA, Immunocytochemistry, Flow Cytometry and Western Blot.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 2.5ug/10e6 cells using MDA-MB-231human breast cancer cell line Western Blot: 0.1ug/mlImmunocytochemistry: 5-15ug/ml using immersion fixed MDA-MB-231 human breast cancer cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months after receipt at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Glypican 1. In Direct ELISA and Western Blot shows ~15% crossreactivity with recombinant mouse Glypican 1 and <1% crossreactivity with recombinant human Glypican 5 and 6.
Isotype：IgG
Calc Applications Abbrev：E FC IC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa24-530 from human Glypican 1, expressed in NSO cells (P35052).
