FPRP (F2alpha Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130kD type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules.
It associates with the 7-TM prostaglandin F2alpha receptor, inhibiting its activity.
It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton.
Mature mouse FPRP is 858 amino acids (aa) in length.
Its extracellular domain (ECD) contains six C2-type Ig-like domains (aa22-813) plus a Pro-Thr-Glu-Leu ER retention signal.
The ECD of mouse FPRP (aa22-832) shares 89% and 98% aa identity with the ECD of human and rat FPRP, respectively.
Applications:Suitable for use in Western Blot and Flow Cytometry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.2ug/ml The detection limit is ~5ng/lane under non-reducing and reducing conditions
Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 3T3-L1 cells in a total reaction volume ≤ 200ul.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile Tris buffered saline, 0.1% BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 1ml sterile Tris buffered saline, 0.1% BSA.
