ESGP (ES cell and germ cell-specific protein) is a presumably secreted polypeptide that belongs to no known protein family.
It is expressed specifically in pluripotential cell types, and does not appear to be involved in differentiation.
Mouse ESGP precursor is 84 amino acids (aa) in length.
It contains a 25 aa signal sequence with a 59 aa mature segment.
There are no N-linked glycosylation sites.
One potential 108 aa variant shows an alternate start site 24 aa upstream of the standard start site.
Applications:Suitable for use in Direct ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml detects ESGP in lysates of D3 mouse embryonic stem cell lineImmunocytochemistry: 5-15ug/ml in immersion fixed D3 mouse embryonic stem cellImmunohistochemistry: 5-15ug/ml in perfusion fixed frozen sections of mouse testisOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul PBS.
Specificity：Recognizes mouse ESGP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Leu24-Lys84 from mouse ESGP, expressed in E. coli. Uniprot/Swiss Accession: Q2Q5T5
