84-1097-63　Endomucin 100ug　E2291-34

特徴

• Endomucin (endothelial sialomucin; also Endomucin-1/2 and Mucin-14) is an 80-120kD glycoprotein member of the Endomucin family of proteins.
• It is expressed on endothelial cells and depending upon its glycosylation pattern, can serve as either a pro- or anti-adhesive molecule.
• Mouse Endomucin precursor is 261 amino acids in length.
• It is type I transmembrane protein that contains a 170 aa extracellular domain (ECD) (aa 21-190) and a 50 aa cytoplasmic region.
• Three splice variants exist in the ECD.
• One shows a deletion of aa 91-141, a second shows a one aa substitution for aa 91-129, and a third shows a one aa substitution for aa 129-142.
• Over aa 21-90, mouse Endomucin shares 60% and 30% aa identity with rat and human Endomucin, respectively.
• Applications:Suitable for use in Flow cytometry, Direct ELISA, Western Blot and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Flow Cytometry: 25ug/mL use 10ul to label 10e6 cellsDirect ELISA: 0.5-1ug/mL detection limit for rmEndomucin ~ 0.3ng/well
• Western Blot: 0.1-0.2ug/mL detection limit for rmEndomucin ~ 1ng/lane under non-reducing and reducing conditions
• Immunocytochemistry: 10ug/mLOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
• Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.