84-1097-62　Dkk-1 (Dickkopf 1, Dickkopf 1-like, Dickkopf Homolog 1, Dickkopf-related Protein 1, DKK 1, hDkk 1, SK) 100ug　D3810-02C

特徴

• Dickkopf (DKK) family of proteins consist of four members Dkk-1, Dkk-2, Dkk-3 and Dkk-4 and function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5 and LRP6.
• Dkks contain two cysteine-rich domains in which the positions of 10 cysteine residues are well conserved.
• Their expression is both temporally and spatially regulated during animal development.
• DKKs also bind with high affinity to transmembrane proteins Kremen1 and 2, which themselves also modulate Wnt signaling.
• Dkk-1 was initially identified as an inducer of head formation in Xenopus embryos and plays an important role in the regulation of bone mass.
• Increased levels of Dkk-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while Dkk-1 expression is decreased in malignant melanoma and colorectal cancers.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:Direct ELISA: 0.5-1ug/m.
• The detection limit is ~0.2ng/well
• Western Blot: 0.1-0.2ug/ml.
• The detection limit is ~10ng/lane under non-reducing and reducing conditions
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.