DC-SCRIPT (dendritic cell-specific transcript; also ZNF366) is an 85kD member of the Cys-Cys:His-His subfamily of zinc-finger proteins.
This subfamily is principally involved in protein-DNA interactions.
DC-SCRIPT appears to be DC specific, and is expressed in myeloid DC, plasmacytoid DC and Langerhans cells.
Human DC-SCRIPT is 744 amino acids (aa) in length.
It contains an R-K-R-K NLS (aa 77-80), a Pro-rich region (aa 110-219), eleven C2H2-type zinc-fingers (aa 255-556) and an extended acidic region (aa 586-698).
Over aa 559-744, human DC-SCRIPT is 68% aa identical to mouse DC-SCRIPT.
Applications:Suitable for use in Flow Cytometry, Direct ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 10ug/ml use 10ul to label 10e6 cells.
Direct ELISA: 0.5-1.0ug/ml detection limit for rhDC-SCRIPT is ~ 0.3ng/well
Western Blot: 0.1-0.2ug/ml detection limit for rhDC-SCRIPT is ~ 5ng/lane under non-reducing and reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
