84-1097-58　CTBS (Chitobiase, Di-N-acetylchitobiase) 100ug　C7947-28

特徴

• CTBS (Di-N-acetylchitobiase; also chitobiase) is a 40kD lysosomal glycoprotein that belongs to glycosyl hydrolase family # 18.
• It is a reducing-end glycoside hydrolase that serves to degrade asparagines-linked carbohydrates.
• Mature human CTBS is 347 amino acids (aa) in length and contains one catalytic domain (aa 115-357).
• There are multiple potential isoforms of CTBS.
• One shows a three aa deletion of aa 30-32, accompanied by a two two aa substitution for the C-terminal 279 amino acids.
• There are two potential alternate start sites, one at Met92 and a second at Met207.
• Mature human CTBS (aa 39-385) is 85% aa identical to mouse CTBS.
• Applications:Suitable for use in Direct ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Direct ELISA: 0.5-1.0ug/ml detection limit for rhCTBS is ~ 0.2ng/well
• Western Blot: 0.1-0.2ug/ml detection limit for rhCTBS is ~ 10ng/lane under non-reducing and reducing conditions
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.