Complement Factor H-related protein 1 is a 43kD, secreted member of the factor H family of glycoproteins.
It is produced by hepatocytes and circulates as two differentially glycosylated isoforms (37kD and 43kD).
Mature human FHR1 is 312aa in length.
It contains five, ~60aa SCRs (short consensus repeats/CCPs/SUSHI repeats) that basically constitute the entire molecule.
FHR1 may play a role in lipoprotein complexes that bind PMNs to LPS.
There is no reported rodent counterpart to FHR1.
Over aa 19-143 of the FHR1 precursor, human FHR2 and FHR5 show 98% and 86% aa identity, respectively.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1ug/ml with recombinant Complement Factor H-Related Protein 1 Immunoprecipitation: 25ug/ml.
Used to immunoprecipitate recombinant human FHR1 from conditioned media of transfected NSO cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Complement Factor H-Related Protein 1 (FHR1). Shows ~25% crossreactivity with recombinant human FHR2 and ~15% crossreactivity with recombinant human FHR5.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Glu19-Ala328 from human Complement Factor H-Related Protein 1 (FHR1), expressed in NSO cells (AAH16755).
