Collagen XXV (Collagen 25 alpha 1 chain; also CLAC-P) is a neuronal, 65-75kD member of the Collagenous transmembrane (TM) subfamily of the Collagen protein family.
It is synthesized as a 654 amino acid (aa) type II TM glycoprotein that contains a 33 aa cytoplasmic region (aa 1-33) and a 600 aa extracellular domain (ECD) (aa 55-654).
The ECD contains seven Collagen-like domains.
Furin cleavage between Arg112-Glu113 generates a 70kD soluble fragment that homotrimerizes and binds to early ABeta amyloid deposits.
There are at least five multiple splice forms that impact the ECD.
Amino acids 139-146 and the seventh Collagen-like domain (aa 571-630) are most often affected.
Over aa 189-654, human COL25A1 is 95% aa identical to mouse COL25A1.
Applications:Suitable for use in Direct ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Direct ELISA: 0.5-1.0ug/ml detection limit for rhCOL25A1 is ~ 0.5ng/well
Western Blot: 1.0ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
