84-1097-55　CHIP (Carboxy Terminus of Hsp70p-interacting Protein, STUB1, STIP1 Homology and U-Box Containing Protein 1, HSPABP2, NY-CO-7, SDCCAG7, Serologically Defined Colon Cancer Antigen 7, Heat Shock Protein A Binding Protein 2 (C-terminus)) 100ug　C4125-03C

特徴

• STUB1 (STIP1 homology and U-box-containing protein 1, also known as C-terminus of Hsc70-interacting protein, CHIP) is a 35kD ubiquitin ligase that participates in intracellular protein folding/refolding and degradation.
• It is highly expressed in striated muscle.
• STUB1 may complex with the molecular chaperones Hsp70 and Hsp90, or act by itself.
• In either event, it regulates protein stability and ubiquitination.
• Human STUB1 is 303 amino acids (aa) in length.
• It contains one TPR domain (aa 27-127) plus a C-terminal U-Box (aa 226-299).
• Its TRP domain interacts with heat shock proteins, while the U-Box participates in ubiquitination.
• There is one isoform that shows an alternate start site at Met73.
• Human and mouse STUB1 are 97% aa identical.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1.0ug/ml
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.