The CILP-1 (cartilage intermediate-layer protein 1) gene product is a monomeric glycoprotein precursor of two secreted, proteolytically generated products, a 90kD N-terminal CILP-1, and a 62kD C-terminal NTPPHase-homolog.
It is found in both hyaline and fibrocartilage.
Although the C-terminal NTPPHase-homolog (aa 725-1184) does not show enzyme activity, it may participate in matrix structural integrity.
Human CILP-1 C-terminus shares 93% and 94% aa identity with porcine and mouse CILP-1 C-terminus, respectively.
Applications:Suitable for use in Direct ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1ug/ml with recombinant human CILP‑1 C-Terminal FragmentOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human CILP-1. Less than 1% cross-reactivity with recombinant human CILP-1 N-terminal peptide is observed.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg724-Asn1184 from human CILP-1, expressed in NS0 cells.
