ASAHL (N-acylsphingosine-amidohydrolase-like; N-acylethanolamine-hydrolyzine acid amidase; also acid ceramidase-like protein) is a 30kD member of the acid ceramidase family.
It is a secreted lysosomal glycoprotein that cleaves fatty acid amides such as palmitoylethanolamine.
Human ASAHL is synthesized as a 359 amino acid (aa) preproprecursor that contains a 28 aa signal sequence, a 96 aa prosegment, and a 30-31kD, 235 aa mature region that begins at Cys125.
Multiple isoforms likely exist.
One isoform shows a deletion of aa 324-359, while another has a two aa substitution for aa 198-359.
The human proform (aa 29-359) is 78% aa identical to mouse ASAHL proform.
Applications:Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Direct ELISA: 0.5-1.0ug/ml detection limit for rhASAHL is ~ 1ng/well
Western Blot: 0.1-0.2ug/ml detection limit for rhASAHL is ~ 10ng/lane under non-reducing and reducing conditions.
Immunoprecipitation:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile 40-50% glycerol, PBS.
