Apolipoprotein C2 (ApoC2/II) is a 8-9kD, secreted member of the Apolipoprotein C2 family of proteins.
It is produced by hepatocytes and represents a major component of VLDL particles.
It activates lipoprotein lipase and may self-associate to form amyloid-type fibrils.
The human ApoC2 precursor is 101 amino acids (aa) in length.
It contains a 22 amino acid (aa) signal sequence, followed by a 79 aa ProApoC2 that contains a lipid-binding region (aa 43-51) and an enzyme interaction site (aa 55-78).
ProApoC2 represents > 90% of circulating ApoC2.
In human, limited proteolytic processing occurs with removal of the six aa prosegment (aa 23-28).
This does not affect activity.
Human ProApoC2 is 59% aa identical to mouse and dog ProApoC2, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.25ug/mLOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS containing 50ug of bovine serum albumin (BSA). Reconstitute with 200ul TBS, 0.1% BSA. Labeled with Biotin.
Specificity：Recognizes human Apolipoprotein C2.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：E. coli-derived recombinant human Apolipoprotein C2, Thr23-Glu101.
