G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling.
Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization.
The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases.
The catalytic domain is flanked by an amino-terminal RGS domain of 183-188 amino acids and a carboxyl-terminus of variable length.
GRK2 is a member of the BetaARK subfamily, and was originally termed Beta-adrenergic receptor kinase 1 (BetaARK1).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/ml in lysates of HeLa human cervical epithelial carcinoma cell line, BaF3 mouse pro-B cell line, and Rat-2 rat embryonic fibroblast cell line.
Immunohistochemistry: 5-15ug/ml in immersion fixed paraffin-embedded sections of human Alzheimer's disease brainKnockout Validated: GRK2 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in GRK2 knockout HEK293T cell line
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes endogenous human GRK2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB KO
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to Pro468-Leu689 human GRK2, expressed in E. coli.
