84-1097-42　ADAMTS4 (Aggrecanase I, A Disintegrin And Metalloproteinase with Thrombospondin motifs 4) 100ug　A0859-60B

特徴

• ADAMTS4 is a member of the family of secreted zinc proteases with a multi-domain structure (1-3).
• The protein precursors consist of a signal peptide and the following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich and spacer.
• It is the only ADAMTS identified that has one TS type I motif.
• It is an active protease effectively cleaving alpha-2-macroglobulin and aggrecan at multiple sites, and is inhibited by TIMP-3 with inhibition constants in subnanomolar range (4-6).
• It receives great attention due to the elevation in its mRNA level after treatment with Interleukin-1 (7).
• However, in a mouse model of osteoarthritis, ADAMTS4 knock-out mice did not exhibit any significant protective effect (8).
• The purified recombinant human ADAMTS4 starts at the catalytic domain and ends before the spacer domain.
• If desired, the aggrecanase activity can be inhibited by 5mM 1,10-phenanthroline and recombinant human TIMP-3.
• Applications:Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.1ug/mlImmunoprecipitation: 25ug/ml immunoprecipitates ADAMTS4 from conditioned cell culture medium spiked with recombinant human ADAMTS4
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.