Allergy to chicken egg or proteins is one of the more frequent causes of food hypersensitivity in infants and young children.
Both IgG and IgA class antibodies may be detected.
Ovalbumin intolerance has been implicated in a number of conditions affecting children.
In particular, children with cystic fibrosis show elevated anti-ovalbumin antibodies.
Ovalbumin antibodies havealso been noted in some forms of kidney disease.
Intolerance to egg proteins could be due not only to the ovalbumin protein found in egg white but also to other major proteins present in the yolk.
The major proteins of chicken eggs are: Ovalbumin (45kD, 54%), Conalbumin (13%, 80kD), Ovomucoid(11%, 28kD), Lysozyme (3.5%, 14kD), Globulins (G2, G3) (8.0%, mol wt), Ovomucin (1.5%, mol wt).
Other protein components include, flavoprotein (0.8%), ovoglycoprotein (0.5%), ovomacroglobulin (0.5%), ovoinhibitor (0.l%) and avidin (0.05%).
Egg allergies occur in about 0.5 percent of the population and in about 5 percent of children with allergies.
Because influenza and yellow fever vaccines are both made in eggs, egg proteins (primarily ovalbumin) are present in the final product.
Residual quantities of egg proteins found in the influenza vaccine (i.e., about 0.02-1ug per dose) are sufficient to induce severe but rarely fatal hypersensitivity reactions in children with egg allergies.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:50,000Western Blot: 1:1000-5000 (ECL)Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：chicken
Grade：Serum
Purity：Serum
Form：Supplied as lyophilized serum containing sodium azide. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes chicken egg white and yellow proteins.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Ch
Immunogen：Chicken egg total proteins (egg white and yolk).
