84-1097-28　FASN (Fatty Acid Synthase) 100ul　F0020-11

特徴

• Fatty Acid Synthase (FASN) catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids.
• In some cancercell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FASN is fused in-frame with the C-terminus of ER-alpha.
• FASN ishighly expressed in adipocytes and liver.
• FASN is a central enzyme in de novo lipogenesis.
• FASN is a target for SREBP and is upregulated by LXR activation; it is also one of the accepted markers for insulin resistance, SREBP and LXR activation.
• FASN: Bovine: 2513 aa; Pig: 2411 aa; Chick: 2511 aa; Mouse: 2504 aa; Rat: 2505 aa; Human: 2511 aa; 273.4kD; 17q25.
• Expression: Ubiquitous; more prominent expression in brain, lungs, and liver.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-10mg/ml chemiluminescence technique
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.