Fatty acid desaturases (FADS) are enzymes that convert a single bond between two carbon atoms (C–C) to a double bond (C=C) in a fatty acyl chain.
The resultant double bond is often referred to as an unsaturated bond, and the reactions catalyzed by these enzymes are known as desaturation reactions.
These reactions require molecular oxygen and occur under aerobic conditions.
The distribution of fatty acid desaturases is almost universal.
The enzyme has been found in all organisms examined, with the exception of some bacteria such as Escherichia coli
There are three types of fatty acid desaturase: FADS1, FADS2 and FADS3.
FADS2 encodes a deduced 444-amino acid protein that shares a high degree of homology with FADS2 and FADS3.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilutions:ELISA: 1:100,000 using 50-100ng control peptide/wellWestern Blot: 1-10mg/ml chemiluminescence technique
Optimal dilutions to be determined by the researcher.
Control Peptide:F0020-06: FADS2 Mouse, Control Peptide (Fatty Acid Desaturase2)
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 0.1% BSA. Reconstitute with 100ul of sterile PBS.
Specificity：Recognizes mouse FADS2.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to 20aa near the N-terminal of mouse FADS2 conjugated to KLH (Q9Z0R9). Species Sequence Homology: rat; 95%, monkey, bovine and rabbit; 80%, human and chimp; 70%.
