84-1097-24　FADS1 (Fatty Acid Desaturase1) 100ug　F0020-03

特徴

• Fatty acid desaturases (FADS) are enzymes that convert a single bond between two carbon atoms (C–C) to a double bond (C=C) in a fatty acyl chain.
• The resultant double bond is often referred to as an unsaturated bond, and the reactions catalyzed by these enzymes are knownas desaturation reactions.
• These reactions require molecular oxygen and occur under aerobic conditions.
• The distribution of fatty acid desaturases is almost universal.
• The enzyme has been found in all organisms examined, with the exception of some bacteria such as Escherichiacoli.
• There are three types of fatty acid desaturase: FADS1, FADS2 and FADS3.
• FADS1 encodes a deduced 444-amino acid protein that shares a high degree of homology with FADS2 and FADS3.
• FADS1: Rat: 447 aa; Human: 444 aa; Mouse: 447 aa; ~52kD; Chromosome: 19A
• Highly expressed in cerebrum, liver and bone.
• Applications:Suitable for use in ELISA.
• Western Blot, though not tested, may potentially be used as an application.
• Other applications not tested.
• Recommended Dilutions:ELISA: 1:100,000 using 50-100ng control peptide/wellOptimal dilutions to be determined by the researcher.
• Control Peptide:F0020-04: FADS1 Mouse, Control Peptide (Fatty Acid Desaturase1)
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.