Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis.
Actin is one of the most conserved cellular protein.
At least 6 actin isoforms have been identified by protein sequence analyses.
Four actin isoforms represent the differentiation markers of muscle tissues.
There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one bactin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle).
Actin isoform are >90% conserved, except in the N-terminal 18-aa (50-60% homology).
Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1: 50,00 Control peptide can be used to coat ELISA plates at 1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute in 100ul PBS.
Specificity：Recognizes human Beta-Actin. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu Mo Rt
Immunogen：Purified (>95%) human platelet (nonmuscle) beta-actin protein.
