Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions: Immunohistochemistry: 2.5ug/mlWestern Blot: 1:100-1:1000ELISA: 1:3000-1:12,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:50ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity:Recognizes Human Sprouty (Drosophila) Homolog 4 (SPRY4). Species Crossreactivity: bovine, canine, mouse, rat.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Bo Ca Hu Mo Rt
Immunogen:Synthetic peptide, KLH-conjugated. Epitope: Amino acids 306-322 of Human Sprouty-4 protein.