Applications:Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions: Immunohistochemistry: 2.5ug/mlWestern Blot: 1:2000-1:20,000ELISA: 1:25,000-1:100,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes Human SMAD, Mothers Against DPP Homolog 3 (SMAD3). Species Crossreactivity: Bovine, Chicken, Frog, Human, Mouse, Pig, Rat, Zebrafish.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Bo Ch Fr Hu Mo Po Rt Ze
Immunogen：Synthetic peptide, KLH-conjugated. Epitope: Amino acids 417-425 of human SMAD3 protein.
