Myosin is the major component of thick muscle filaments and is a long asymmetric molecule containing a globular head and a long tail.
The molecule consists of two heavy chains each ~200,000D, and four light chains each ~16,000-21,000D.
Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction.
Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain.
The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells.
This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site.
Human, mouse and rat have almost identical sequences.
Applications:Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions: Western Blot: 1:500-1:2000ELISA: 1:10,000-1:70,000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes human Myosin. Species Crossreactivity: mouse and rat. Detects both unphosphorylated and monophosphorylated forms of the protein.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids aa10-35 of human myosin light chain protein.
