Muscarinic receptor M3 is a G protein-coupled receptor for acetylcholine which is expressed in smooth muscle and glandular tissues.
The M3 subtype is coupled to other G proteins known as Go or Gq which activate phospholipase C leading to an increase in intracellular Ca2+.
The M3 muscarinic receptor controls smooth muscle contraction in blood vessels and lungs as well as in the gastrointestinal tract.
It is also located in many glands, and its activation helps to stimulate secretion.
Mutations in the CHRM gene can lead to the development of Prune Belly Syndrome (PBS), a condition characterized by weak abdominal musculature, lax overlying skin, cryptorchism and urinary tract abnormalities (Weber et al.
2011).
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 10ug/ml Requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections.
0.01M sodium citrate buffer, pH 6.0 is recommended for this purpose
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes Human Muscarinic Acetylcholine Receptor M3 (CHRM3).
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the 3rd cytoplasmic domain of human muscarinic receptor M3 (KLH). Species sequence homology: mouse.
