Applications:Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions: Immunohistochemistry: 5ug/mlWestern Blot: 1:500-1:2000ELISA: 1:10,000-1:50,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:50ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity:Recognizes Human DNA Replication Licensing Factor Mcm2 (MCM2). Species Crossreactivity: Mouse, Rat, yeast.
Isotype:IgG
Calc Applications Abbrev:E IF IHC WB
Calc Crossreactivity:Hu Mo Rt Ye
Immunogen:Synthetic peptide, KLH-conjugated. Epitope: Amino acids 21-31 of human MCM2 protein (see below).