Applications:Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1.25ug/ml, Requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections.
ELISA: 1:6000-1:30,000Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human Gli3. Species Crossreactivity: Chimpanzee, canine, chicken, quail, squirrel monkey and xenopus
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Ch Fr Hu Mk Xe
Immunogen：Synthetic peptide corresponding to an internal region near amino acids 30-60 of human Gli-3.
