Applications:Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 2.5ug/mlWestern Blot: 1:250-1:2000ELISA: 1:4000-1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:50ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity:Recognizes human FK506 Binding Protein 12-rapamycin Associated Protein 1 (FRAP1). Species Crossreactivity: canine, mouse, rat, zebrafish.
Isotype:IgG
Calc Applications Abbrev:E IF IHC WB
Calc Crossreactivity:Ca Hu Mo Rt Ze
Immunogen:Synthetic peptide, KLH-conjugated. Epitope: Amino acids 2440-2457 of human mTOR.