84-1094-78　［取扱停止］ADAM 17 (A Disintegrin And Metalloproteinase 17, TNF-a Converting Enzyme, TACE, TNF alpha Converting Enzyme) 50ug　A0859-35J

特徴

• Tumor-necrosis factor-a is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death.
• TNF-a is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-a.
• TNF-a converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately.
• TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease).
• TACE also processes other cell surface proteins, including TNF receptor, TGFa, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP).
• TACE mRNA is expressed in a variety of human and murine tissues.
• TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.
• ADAM17 is a Metallopeptidase M12B type protease.
• Applications:Suitable for use in Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 20ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.