Alpha Synuclein is a protein of 140aa expressed abundantly in the brain.
a-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites.
Mutations of the a-synuclein gene have been linked to Parkinson disease.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000; Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Positive Controls:HeLa, 293, rat brain and mouse brain cell extracts.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human alpha-Synuclein at ~18kD. May crossreact with beta synuclein. Species Crossreactivity: rat, mouse and monkey.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to the C-terminal sequence of human alpha-Synuclein
