alpha-internexin is a ~66 kD Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits.
It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization.
It is expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes.
Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M anbd NF-H.
In some mature neurons alpha-internexin is the only neurofilament subunit expressed.
Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture.
In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:10,000Immunohistochemistry: 1:1000Immunocytochemistry: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：chicken
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid.
Specificity：Recognizes human alpha-Internexin. Species Crossreactivity: mouse and rat.
