84-1094-57　［取扱停止］MAP2 (Microtubules Associated Protein 2) 50ul　M2350-01H

特徴

• Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein tau and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5.
• MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b.
• A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels.
• All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau.
• MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells.
• Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites.
• In contrast tau is found predominantly in neuronal axons.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:100,000Immunohistochemistry: 1:10,000Immunocytochemistry: 1:10,000Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1906IgY, Chicken Pab Rb x Ch I1906-10IgY, Chicken (HRP) Rb x Ch I1906-12IgY, Chicken (FITC) Rb x Ch I1906-14IgY, Chicken (AP) Rb x Ch
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.