Glial fibrillary acidic protein (GFAP) is the 10nm or intermediate filament protein found specifically in astrocytic cells in the central nervous system.
GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves.
In many damage and disease states GFAP expression is heavily upregulated in astrocytes.
In addition neural stem cells frequently strongly express GFAP.
Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:5000Immunohistochemistry: 1:1000-1:5000Immunocytochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1906-10:IgY, Chicken (HRP) Rb x Ch I1906-12:IgY, Chicken (FITC) Rb x Ch I1906-14:IgY, Chicken (AP) Rb x Ch
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：chicken
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid, 5mM sodium azide.
Specificity：Recognizes human GFAP. Species Crossreactivity: feline, mouse, rat and primate.
Isotype：IgY
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Fe Hu Mo Rt
Immunogen：Full-length, recombinant protein corresponding to human GFAP isotype 1, expressed in E. coli.
