Neuropeptide Y is one of the most abundant neuropeptides in the mammalian nervous system and exhibits a diverse range of important physiologic activities, including effects on psychomotor activity, food intake, regulation of central endocrine secretion, and potent vasoactive effects on the cardiovascular system.
Two major subtypes of NPY (Y1 and Y2) have been defined by pharmacologic criteria.
NPY receptors, such as NPY1R, have been identified in a variety of tissues, including brain, spleen, small intestine, kidney, testis, placenta, and aortic smooth muscle.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:500-1:1000Immunocytochemistry: 1:500Indirect Immunofluorescence: strong positive labeling of rat cortex, arcuate and hippocampus by using biotin/avidin HRP detection
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.
Specificity：Recognizes rat NPY 1R at ~40kD. Species Crossreactivity: human, mouse.
Isotype：IgG
Calc Applications Abbrev：IC IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa356-382 of the rat NPY Y1 receptor
