84-1094-22　［取扱停止］NORE1 (New Ras Effector 1, NORE1A, NORE1B, Maxp1, Ras Association Domain Containing Protein 5, RASSF5, Regulator for Cell Adhesion and Polarization Enriched in Lymphoid Tissues, RAPL) 100ug　N3945-01

特徴

• RASSF1A, a new tumor-suppressor gene located at 3p21.3 is frequently inactivated by promoter region hypermethylation in a variety of human cancers including lung, breast, kidney and neuroblastoma.
• Another member of the RASSF1 gene family, Ras effector NORE1, which is structurally related to RASSF1, can also mediate a Ras-dependent apoptosis.
• Moreover, an analysis of NORE1 protein expression showed that it is frequently down-regulated in lung tumor cell lines and primary lung tumors.
• The RAS oncogene is mutated in nearly one third of all human cancers.
• Members of the RAS superfamily are plasma membrane GTP binding proteins that modulate intracellular signal transduction pathways.
• A subfamily of RAS effectors, including NORE1, share a RAS association (RA) domain.
• NORE1 mediates a Ras dependent apoptosis.
• NORE1 exists in at least two spliced isoforms, A and B.
• Transcript A encodes a protein of 418 aa (~47kD) while transcript B contains an ORF of 265 aa (~30.5kD).
• NORE1A and NORE1B are expressed in most of the normal tissues analysed and are potential tumor suppressors.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.