CD39 is an integral membrane protein with two transmembrane domains and a large extracellular region (Maliszewski et al, 1994) with nucleoside triphosphate diphosphohydrolase activity (Wang and Guidotti, 1996).
CD39 hydrolyzes nucleotide substrates at extremely high turnover rates, converting ATP directly into AMP without releasing ADP (Wang and Guidotti, 1998).
CD39 has six putative N-glycosylation sites within its sequence (Maliszewski et al, 1994) and is heavily glycosylated (Wang et al, 1998).
Wu et al (2005) have reported that the presence or absence of certain N-linked oligosaccharides on CD39 affect its enzymatic activity.
In the plasma membrane, CD39 forms oligomers that are essential for its enzymatic activity (Wang et al, 1998) CD39 functions on the outer face of the plasma membrane (Maliszewski et al, 1994; Wang and Guidotti, 1996, 1998; Marcus et al, 1997; Wang et al, 1997; Koziak et al, 2000) and is not active until it reaches the plasma membrane.
Complete N-glycosylation of CD39 correlates with its enzymatic activity (Zhong et al, 2001).
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：guinea pig
Source Antibody：rat
Grade：Serum
Purity：Serum
Form：Supplied as a liquid, 0.05% sodium azide.
Specificity：Recognizes rat CD39.
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to DAGSSHTNLYIY
