VSV is a member of the Rhabdoviridae family, and consists of a single RNA molecule that encodes five proteins, one of which is a surface glycoprotein (G).
G is a type I membrane glycoprotein and is responsible for viral binding to cell surface receptors and initiating the infectious process.
VSV-G Epitope Tags specifically recognize fusion proteins containing the VSV-G epitope tag sequence (YTDIEMNRLGK).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.02-0.1ug/mlWestern Blot: 0.1-1.0ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 1% BSA and 0.01% thimerosal. Labeled with horseradish peroxidase (HRP).
Specificity：Binds to over-expressed fusion proteins containing a VSV-G epitope tag.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：VSV-G epitope tag peptide YTDIEMNRLGK conjugated to KLH
