Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-0.2ug/mlWestern Blot: 0.05-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:40ug
Host:goat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity:Specific for the Thioredoxin fusion protein from human and E. coli. Reacts with the 109-aa Trx-tag sequence encoded by pET-32a expression vectors. Suitable for detecting fusion proteins that contain a Trx-tag.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Ec Hu
Immunogen:Purified recombinant Trx-tag fusion protein