Streptavidin is a tetrameric protein from Streptomyces avidinii that binds very tightly to the vitamin biotin with a Kd of ≈ 10-14mol/L.
The high-affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
Applications:Suitable for use in ELISA, Western Blot and Dot Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5-1ug/mlWestern Blot: 0.5-1ug/mlDot Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：5mg
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 1ml sterile ddH2O.
