Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses.
NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS.
NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity.
The long-sought IkB kinase (IKK), which phosphorylates IkB, and mediates IkB degradation and NF-kB activation, was recently identified by several laboratories.
IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKa and IKKb).
IKKa and IKKb interact with each other and both are essential for the NF-kB activation.
IKKa specifically phosphorylates IkB-a.
IKKa is expressed in variety of human tissues.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide and 50% glycerol.
Specificity：Detects endogenous levels of total IKKa protein.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthesized non-phosphopeptide derived from human IKKa around the phosphorylation site of threonine 23 (L-G-TP-G-G)
