NFkB exists in the cytoplasm as a complex with a 38kD inhibitor protein, IkB (MAD-3).
Activation of NFkB by different stimuli, including TNFa and PMA, requires the dissociation of the inhibitor protein.
Dissociation is regulated by the phosphorylation of IkB on serine residues 32 and 36 which targets IkB for ubiquinitation and proteasome mediated degradation.
The NFkB complex then translocates to the nucleus and activates transcription of responsive genes.
ApplicationsSuitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability:For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide and 50% glycerol.
Specificity：Detects endogenous levels of IkBa only when phosphorylated at tyrosine 42.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Synthesized phosphopeptide derived from human IkBa around the phosphorylation site of tyrosine 42 (E-E-YP-E-Q)
