Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.
Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.
Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.
This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.
This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.
Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.05-0.5ug/mlWestern Blot: 0.1-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：40ug
Host：goat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.01% thimerosal. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 80ul or 200ul sterile ddH2O.
Specificity：Recognizes over-expressed fusion proteins containing either amino- or carboxy-terminal HA epitope tag.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Synthetic peptide corresponding to YPYDVPDYA (Influenza hemagglutinin-HA (epitope)), conjugated to KLH.
