84-1092-79　［取扱停止］HAT Epitope Tag, KDHLIHNVHKEFHAHAHNK 100ug　H1823-40

特徴

• The HAT Epitope Tag, KDHLIHNVHKEFHAHAHNK, derived from a sequence from chicken lactate dehydrogenase.
• Neutral pH purification is possible due to the unique polyhistidine epitope tag.
• Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.
• Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.
• Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.
• This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.
• This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
• The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.
• Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.05-0.2ug/mlWestern Blot: 0.1-1.0ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.