The glutathione-S-transferases are a family of enzymes that catalyze the addition of the tripeptide glutathione to endogenous and xenobiotic substrates which have electrophilic functional groups.
They play an important role in the detoxication and metabolism of many xenobiotic and endobiotic compounds.
The glutathione adducts produced have increased solubility in water and are subsequently enzymatically degraded to mercapturates and excreted.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.05-0.5ug/mlWestern Blot: 0.1-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity:Recognizes GST protein from Schistosoma japonicum and is useful for detecting GST and GST-fusion proteins. Crossreactivity with cell lysate of E. coli bacteria, sf21 cell and yeast has not been observed.
Isotype:IgG
Calc Applications Abbrev:E WB
Immunogen:Recombinant protein corresponding to Glutathione-S-Transferase from Schistosoma japonicum